Fundamentals of Recombinant Protein Production, Purification and Characterization 1st Edition

Fundamentals of Recombinant Protein Production, Purification and Characterization (1st Edition) by Deepti Yadav, Abhishek Guldhe, Tukayi Kudanga is an essential, practice-oriented guide for scientists, students, and industry professionals working across biotechnology, biopharma, and academic research labs worldwide. Clear, concise, and current, this title bridges molecular biology and bioprocess engineering to demystify every stage of recombinant protein work — from gene design and expression systems to downstream purification and analytical characterization.

Begin with a compelling overview of expression platforms — bacterial, yeast, insect, and mammalian systems — and learn how to select the optimal host for yield, solubility, and post-translational modifications. Detailed chapters on chromatographic techniques, filtration, and formulation provide practical strategies for achieving high purity and activity. Advanced sections explain mass spectrometry, SDS‑PAGE, Western blotting, and biophysical tools used to confirm identity, folding, and stability, making this book invaluable for structural biology and therapeutic development.

Readers gain actionable insights: troubleshooting common expression problems, scaling protocols from bench to pilot scale, and optimizing cost-effective purification workflows for enzymes, antibodies, and fusion proteins. Case studies and comparative tables streamline decision-making for labs in North America, Europe, Asia-Pacific and beyond, helping teams accelerate research and reduce time-to-market.

Whether you’re a graduate student preparing your first recombinant construct or a process scientist designing GMP-compliant purification, this book equips you with the knowledge to produce high-quality proteins reliably. Practical, authoritative, and globally relevant, Fundamentals of Recombinant Protein Production, Purification and Characterization is a must-have reference to advance your projects and strengthen your lab’s capabilities. Order now to bring clarity and confidence to your protein workflows.

Note: eBooks do not include supplementary materials such as CDs, access codes, etc.